do you actually experience any audio issues on your PC while using GlassWire?
When there is strong network activity, yes, alongside the other mentioned issues.
Did Speedtest itself show any changes in speed at all while running GlassWire?
I mentioned this in my previous comment, there was a decrease in download speed while GW was running.
If you run any other background app like an antivirus, or browser does it have the same latency issues?
I only use Windows Defender in the background and the browser is just as affected as any other app on the system, I mentioned YouTube as an example.
GlassWire does use computer resources like most other software, but I wonder if any app that uses many resources can cause audio latency issues?
Not to that extent, I can run games at settings that make my FPS single digits and things like audio playback will still be fine, this kind of timing issues usually doesn’t happen even with applications using a lot of resources, the CPU scheduling is there to prevent this.
Edit: I also found this thread: Very high DPC routine execution time for TCPIP.SYS with Glasswire 1.20 At least one other person experienced this issue.
Edit 2: I assume “network auto scanning” is the “Things” monitor? I have that already disabled.
Edit 3: What is the download speed you are using for testing? My setup didn’t show symptoms that significantly (although there was still some audio crackling and audio/video desync here and there) before I upgraded to Gigabit.